I-Glutaraldehyde Technological Frontier: Intuthuko kubuchwepheshe bokulwa ne-Calcification

Emkhakheni wokufakelwa kwenhliziyo, i-glutaraldehyde ibilokhu isetshenziswa isikhathi eside ukwelapha izicubu zezilwane (njenge-bovine pericardium) ekukhiqizweni kwama-valve e-bioprosthetic. Kodwa-ke, amaqembu e-aldehyde amahhala asele avela ezinqubweni zendabuko angaholela ekufakweni kwe-calcification ngemuva kokufakelwa, okubeka engcupheni ukuqina kwesikhathi eside kwemikhiqizo.

Ukubhekana nale nselele, ucwaningo lwakamuva olwanyatheliswa ngo-Ephreli 2025 lwethula ikhambi elisha lokwelapha i-anti-calcification (igama lomkhiqizo: i-Periborn), elifinyelela intuthuko emangalisayo.

1. Ukuthuthukiswa Kobuchwepheshe Okuyinhloko:

Lesi sixazululo sethula ukuthuthukiswa okuningana okubalulekile enkambisweni yokuxhumanisa yendabuko ye-glutaraldehyde:

Ukuxhumanisa Isinyibilikisi Semvelo:
Ukuxhumanisa i-Glutaraldehyde kwenziwa ku-solvent ephilayo eyakhiwe yi-ethanol engu-75% + 5% ye-octanol. Le ndlela isiza ekususeni ngempumelelo ama-phospholipid ezicubu ngesikhathi sokuxhumanisa i-cross-linking—ama-phospholipid ayizindawo eziyinhloko ze-nucleation ze-calcification.

I-ejenti Yokugcwalisa Isikhala:

Ngemva kokuxhumanisa, i-polyethylene glycol (PEG) isetshenziswa njenge-ejenti yokugcwalisa isikhala, ingena ezikhaleni eziphakathi kwemicu ye-collagen. Lokhu kokubili kuvikela izindawo ze-nucleation zamakristalu e-hydroxyapatite futhi kuvimbele ukungena kwe-calcium ne-phospholipids ku-plasma yomnikazi.

Ukuvalwa Kwesiphetho:

Ekugcineni, ukwelashwa nge-glycine kuqeda amaqembu e-aldehyde asele, asabela kalula, ngaleyo ndlela kususwe esinye isici esibalulekile esibangela i-calcification kanye ne-cytotoxicity.

2. Imiphumela Evelele Yezokwelapha:

Lobu buchwepheshe busetshenziswe esicabheni senhliziyo senkomo esibizwa ngokuthi “i-Periborn.” Ucwaningo lokulandelela oluhlanganisa iziguli ezingu-352 eminyakeni engaphezu kwengu-9 lubonise ukukhululeka ekusetshenzisweni kabusha ngenxa yezinkinga ezihlobene nomkhiqizo ezifinyelela ku-95.4%, okuqinisekisa ukusebenza kahle kwaleli cebo elisha lokulwa ne-calcification kanye nokuqina kwalo okumangalisayo kwesikhathi eside.

Ukubaluleka Kwalokhu Kuphumelela:

Akugcini nje ngokubhekana nenselele yesikhathi eside emkhakheni wama-valve e-bioprosthetic, okwandisa isikhathi sokuphila komkhiqizo, kodwa futhi kufaka amandla amasha ekusetshenzisweni kwe-glutaraldehyde ezintweni zokwelapha zezinga eliphezulu.